Single-Cell RNA Sequencing

A sequencing-based method that measures gene expression profiles at single-cell resolution by isolating individual cells or nuclei, reverse-transcribing their RNA to cDNA, adding cell-specific barcodes, sequencing the resulting libraries, and assigning reads to genes per cell to produce a cell-by-gene expression matrix.

A genomics method that profiles the transcriptome of individual cells by isolating single cells or nuclei, barcoding their RNA, and sequencing resulting libraries to measure gene expression at single‑cell resolution and reveal cellular heterogeneity.