High-Performance Liquid Chromatography

A liquid-phase chromatographic technique that uses elevated pressure to drive a mobile phase through a tightly packed column containing a stationary phase, producing high-resolution separation of mixture components for qualitative and quantitative analysis.

A chromatographic separation technique in which a liquid mobile phase is pumped at elevated pressure through a packed column containing a stationary phase so that components of a dissolved sample separate based on differential interactions (partitioning, adsorption, size exclusion, ion exchange, etc.); separated analytes are detected and quantified by on-line detectors.