##  [Yeast Two-Hybrid Assay](/yeast-two-hybrid-assay) 

  ##  [Yeast Two-Hybrid Assay](https://natural.quantumdictionary.io/yeast-two-hybrid-assay-0) 

  

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**Natural & Formal Sciences Dictionary**

 







 

 

 

 



 

 

 

 

Definition

A genetic assay that detects binary protein–protein interactions by expressing two fusion proteins in yeast: one fused to a DNA-binding domain (bait) and the other to a transcriptional activation domain (prey); physical interaction reconstitutes a functional transcription factor that drives a reporter gene.